GSM7429711: F4R5Sept2017; Mus musculus; RNA-Seq - SRA

SRX20519091: GSM7429711: F4R5Sept2017; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina MiSeq) run: 222,919 spots, 93.3M bases, 59.4Mb downloads

External Id: GSM7429711_r1

Submitted by: INSERM U1236-MOBIDIC

Study: Immunoglobulin repertoires in mice carrying a knock-in human Cµ gene downstream of the IGH 3' regulatory region

PRJNA976398 • SRP439631 • All experiments • All runs

show Abstracthide Abstract

To in-depth analyze the repertoire of B cells after the occurrence of recombination events joining SÙ to the IgH downstream regulatory region (3'RR) Overall design: Spleen B cell RNA samples from knock-in mice, comparing the endogenous IgM µ heavy chain transcripts (VDJ spliced onto MmCµ), versus endogenous mouse spliced IgG2b gamma heavy chain transcripts (VDJ spliced to MmG2b), versus spliced VDJ-hCµ transcripts obtained after recombination close to the ectopic knock-in human Cµ gene

Sample: F4R5Sept2017

SAMN35369333 • SRS17829044 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM7429711

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RACE

Layout: PAIRED

Construction protocol: Total RNA was extracted using TRIzol reagent (ThermoFisher) from 5 millions of CD43- spleen B cells For each sample, 500 ng total RNA was reverse-transcribed to cDNA by a 5'RACE PCR using ProtoScript II (New England Biolabs) and a cap race primer associated with consensus reverse primers for each mouse IgH Cm, Cg and Ca CH1 region or the human Cµ CH1

Runs: 1 run, 222,919 spots, 93.3M bases, 59.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR24741799	222,919	93.3M	59.4Mb	2023-06-28

ID:: 27942774

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