GSM7416805: R1021.11.C241_NKX_2; Mus musculus; RNA-Seq - SRA

SRX20488075: GSM7416805: R1021.11.C241_NKX_2; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 35.8M spots, 5.4G bases, 1.9Gb downloads

External Id: GSM7416805_r1

Submitted by: Maitra laboratory, Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center

Study: Transcriptomic profiles after Nkx6-2 overexpression in IPMN-associated cancer cells derived from murine pancreas [bulk RNA-Seq]

PRJNA975570 • SRP439162 • All experiments • All runs

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To investigate transcriptomic changes associated with Nkx6-2, we overexpressed Nkx6-2 in murine IPMN-associated pancreatic cancer cells by lentivirus transduction and performed bulk RNA-sequencing Overall design: We performed differential gene expression analysis and geneset enrichment analysis using data obtained from RNA-seq of 3 different cell lines with two groups, control and Nkx6-2 overexpression

Sample: R1021.11.C241_NKX_2

SAMN35339793 • SRS17798822 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM7416805

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted using the RNeasy Mini Kit (QIAGEN). 1 ug of total RNA was used for the construction of sequencing libraries. ribosomal RNA depletion was performed using KAPA RiboErase kit (Roche) and libraries were prepared using KAPA Hyperprep kit (Roche) following manufacturer's protocols.

Runs: 1 run, 35.8M spots, 5.4G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR24709422	35,782,740	5.4G	1.9Gb	2023-06-07

ID:: 27911477

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