GSM7300340: A549 Mock-infected, 48 hpi, polyphosphatase-treated, 2; H... - SRA

SRX20233043: GSM7300340: A549 Mock-infected, 48 hpi, polyphosphatase-treated, 2; Homo sapiens; miRNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 17.4M spots, 1.7G bases, 526.6Mb downloads

External Id: GSM7300340_r1

Submitted by: Buck Lab, Institute of Immunology & Infection, The University of Edinburgh

Study: Respiratory Syncytial virus (RSV) directly binds host microRNAs and de-regulates host gene targets [miRNA-seq]

PRJNA967678 • SRP436118 • All experiments • All runs

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To investigate how RSV affects miRNA expression, we performed small RNA-seq at of 48 hrs of viral infection We also compared polyphosphatase-treated samples to non-polyphosphatase-treated samples to identify tri-phosphorylated small RNAs Overall design: small RNA-seq data of A549 cells infected with RSV or mock-treated after 48 of infection

Sample: A549 Mock-infected, 48 hpi, polyphosphatase-treated, 2

SAMN34718125 • SRS17567390 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM7300340

Instrument: Illumina NovaSeq 6000

Strategy: miRNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: RNA was extracted using miRNeasy kit (Qiagen), 1ug of extracted RNA was polyphosphatase-treated and 100ng were used as input for library preparation Libraries were generated using the Trilink CleanTag small RNA library kit according to manufacture's instructions

Runs: 1 run, 17.4M spots, 1.7G bases, 526.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR24445820	17,351,637	1.7G	526.6Mb	2024-05-29

ID:: 27653409

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