GSM7256668: TCF1+CD8+ T cells, no beads, IL-2, no PGE2, rep4; Mus mus... - SRA

SRX20143758: GSM7256668: TCF1+CD8+ T cells, no beads, IL-2, no PGE2, rep4; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 39.5M spots, 11.8G bases, 3.8Gb downloads

External Id: GSM7256668_r1

Submitted by: Division of Animal Physiology and Immunology, Technical University of Munich

Study: RNA sequencing of murine in vitro differentiated and repetitively activated, antigen-experienced TCF1+CD8+ T cells after PGE2 treatment and stimulation [bulk RNA-Seq]

PRJNA963167 • SRP435147 • All experiments • All runs

show Abstracthide Abstract

RNA sequencing was used to characterise PGE2-mediated changes in the gene expression profile of in vitro differentiated and repetitively activated antigen-experienced TCF1+CD8+ T cells. Overall design: In vitro differentiated and repetitively activated antigen-experienced TCF1+CD8+ T cells were pre-incubated with or without PGE2 (100 ng/mL) for 1 hour at 37°C. Subsequently, the cells were stimulated for an additional 4 hours with low dose IL-2 (85 U/ml) or the combination of low dose IL-2 and aCD3/CD28 beads.

Sample: TCF1+CD8+ T cells, no beads, IL-2, no PGE2, rep4

SAMN34436234 • SRS17471953 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM7256668

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was isolated using Total RNA Miniprep (Monarch) Isolated RNA was sent to Novogene for library preparation. Libraries were prepared using mRNA Library preparation Poly A enrichment and The NEB Next® Ultra™ RNA Library Prep Kit (i7 index read, 8bp; i5 index read, 8bp).

Runs: 1 run, 39.5M spots, 11.8G bases, 3.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR24351008	39,457,855	11.8G	3.8Gb	2024-02-21

ID:: 27558985

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity