GSM7207828: psd-13 pollen RNA, IP fraction, replicate 3; Arabidopsis ... - SRA

SRX20032282: GSM7207828: psd-13 pollen RNA, IP fraction, replicate 3; Arabidopsis thaliana; miRNA-Seq
1 ILLUMINA (NextSeq 500) run: 18.9M spots, 963.7M bases, 355.8Mb downloads

External Id: GSM7207828_r1

Submitted by: Biochemistry, University of Otago

Study: Pseudouridine in small RNAs

PRJNA958093 • SRP433690 • All experiments • All runs

show Abstracthide Abstract

Pseudouridine is an isomer of uridine and is the most common RNA modification in both procaryotes and eucaryotes. It is found in ribosomal, transfer, and other structural RNA as well as in some mRNA and non-coding RNA. We have found abundant pseudouridine in small RNA and their precursors in Arabidopsis. Overall design: Identification of pseudouridylated small RNAs and their precursors in Arabidopsis thaliana

Sample: psd-13 pollen RNA, IP fraction, replicate 3

SAMN34276925 • SRS17371576 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Name: GSM7207828

Instrument: NextSeq 500

Strategy: miRNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: tissue ground in liquid nitrogen, Trizol total RNA extraction, 15% urea PAGE fractionation, 17-40nt cut and eluted ON NEBNext small RNA sequencing kit (CMC samples); Perkin Elmer Nextflex v3 (IP samples)

Runs: 1 run, 18.9M spots, 963.7M bases, 355.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR24236138	18,896,147	963.7M	355.8Mb	2023-08-07

ID:: 27431652

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