GSM2250246: Kasumi-3_GATA2_N1; Homo sapiens; ChIP-Seq - SRA

SRX1974321: GSM2250246: Kasumi-3_GATA2_N1; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 40M spots, 1.4G bases, 1.6Gb downloads

Submitted by: NCBI (GEO)

Study: GATA-2 occupancy in Kasumi-3 cell line

PRJNA331135 • SRP079678 • All experiments • All runs

show Abstracthide Abstract

GATA-2 is a master regulator of hematopoiesis which controls expression of multiple genes and is implicated in acute myeloid leukemia (AML). However, the molecular mechanism how GATA-2 deregulation causes leukemogenesis is still unclear. In this study, GATA-2 ChIP-squ analysis was conducted in Kasumi-3 AML cell line to identify GATA-2 target genes which play important roles in the pathogenesis of AML. Overall design: ChIP with GATA-2 antibody was conducted in Kasumi-3 AML cell line and ChIP-seq profile was generated by deep sequencing.

Sample: Kasumi-3_GATA2_N1

SAMN05437877 • SRS1582458 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina Genome Analyzer IIx

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: ChIP-Seq was performed by Peggy J. Farnham at University of Southern California. Immunoprecipitated DNA was processed into Illumina libraries using standard procedures, and libraries were sequenced on Illumina GenomeAnalyzer Iix. Immunoprecipitated DNA was processed into Illumina libraries using standard procedures.

Experiment attributes:

GEO Accession: GSM2250246

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 40M spots, 1.4G bases, 1.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR3949323	39,981,767	1.4G	1.6Gb	2016-08-05

ID:: 2842562

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