GSM2243768: GBM cell 324; Homo sapiens; RNA-Seq - SRA

SRX1963964: GSM2243768: GBM cell 324; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 1.5M spots, 187.7M bases, 71Mb downloads

Submitted by: NCBI (GEO)

Study: Single-Cell RNAseq analysis of diffuse neoplastic infiltrating cells at the migrating front of human glioblastoma

PRJNA330719 • SRP079058 • All experiments • All runs

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We used single-cell RNAseq to investigate the heterogeneity of glioblastoma tumors and assess differential expression between cells within and in proximity of the tumor. Overall design: Examination of cell types in human primary glioblastoma samples.

Sample: GBM cell 324

SAMN05421184 • SRS1573520 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: single-cell sorting in 96-well plates followed by cDNA preparation using Smart-seq2 Smart-Seq2 for preparation of cDNA followed by library preparation using Nextera XT. Nextera tagmentation according to the standard protocol for single cell RNA-seq following Smart-Seq2

Experiment attributes:

GEO Accession: GSM2243768

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 1.5M spots, 187.7M bases, 71Mb

Run	# of Spots	# of Bases	Size	Published
SRR3934672	1,463,595	187.7M	71Mb	2017-11-02

ID:: 2827598

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