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SRX195359: GSM1020006: Smc3 Wapl +/delta rep1; Mus musculus; ChIP-Seq
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 33.1M spots, 1.2G bases, 716.1Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Wapl controls higher-order chromatin structure in interphase chromosomes (ChIP-Seq)
show Abstracthide Abstract
Mammalian genomes contain several billion base pairs of DNA which are packaged in chromatin fibers. At selected gene loci, cohesin complexes have been proposed to arrange chromatin fibers into higher-order structures, but it is poorly understood how cohesin performs this task, how important this function is for determining the structure of chromosomes, and how this process is regulated to allow changes in gene expression. Here we show that the cohesin release factor Wapl controls chromatin structure and gene regulation at numerous loci throughout the mouse genome. Conditional deletion of the Wapl gene leads to stable accumulation of cohesin on chromatin, chromatin compaction, altered gene expression, cell cycle delay, chromosome segregation defects and embryonic lethality. In Wapl deficient chromosomes, cohesin accumulates in an axial domain, similar to how condensins form a “scaffold” in mitotic chromosomes. We propose that Wapl controls chromatin structure and gene regulation by determining the residence time with which cohesin binds to DNA. Overall design: ChIP-Seq using two different antibodies (CTCF, Smc3); one (CTCF) and two (Smc3) replicates; two different genotypes (Wapl +/delta, Wapl -/delta). The control sample is a single-replicate INPUT for each genotype.
Sample: Smc3 Wapl +/delta rep1
SAMN01765667 • SRS369299 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina Genome Analyzer IIx
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Experiment attributes:
GEO Accession: GSM1020006
Links:
External link:
Runs: 1 run, 33.1M spots, 1.2G bases, 716.1Mb
Run# of Spots# of BasesSizePublished
SRR59266633,091,3671.2G716.1Mb2015-07-22

ID:
258370

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