GSM7062064: biol WT1; Mus musculus; RNA-Seq - SRA

SRX19496131: GSM7062064: biol WT1; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 26.8M spots, 8.1G bases, 3.1Gb downloads

External Id: GSM7062064_r1

Submitted by: Emory University

Study: Role of TET1-mediated epigenetic modulation in Alzheimer's disease [RNA-Seq]

PRJNA938432 • SRP424435 • All experiments • All runs

show Abstracthide Abstract

To investigate the influence the partial loss of Tet1 has on AD pathogenesis in 5xFAD mice, we crossed a Tet1 KO mouse line with the 5xFAD mouse line to generate WT, 5xFAD, Tet1(+/-), and FAD/Tet1(+/-) mice. We then performed gene expression profiling analysis using data obtained from RNA-seq from biological replicates of mice comprised of 4 different genotypes. Overall design: Comparative gene expression profiling analysis of RNA-seq data for WT, 5xFAD, Tet1(+/-), FAD/Tet1(+/-) mice to determine how the partial loss of Tet1 influences 5xFAD-associated gene expression and pathology.

Sample: biol WT1

SAMN33436011 • SRS16885966 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM7062064

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: To isolate RNA, mouse cortex was homogenized in 1mL of TRIzol and RNA was purified using chloroform and Isopropyl alcohol Poly(A)+ transcripts were isolated with the NEBNext Poly(A) mRNA Magnetic Isolation Module kit according to the manufacturer's protocol (New England BioLabs Inc., Massachusetts, USA). RNA samples were randomly primed (5' d(N6) 3' [N=A,C,G,T]) and fragmented. The first strand was synthesized with Protoscript II Reverse Transcription with a modified extension period as to what is stated in the manufacturer's protocol (30 minutes at 42⁰C). The remaining steps of library generation were carried out according to the manufacturer's protocol NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA).

Runs: 1 run, 26.8M spots, 8.1G bases, 3.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR23611291	26,759,818	8.1G	3.1Gb	2023-10-03

ID:: 26777097

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