Name: GSM7053939
Instrument: Illumina NovaSeq 6000
Strategy: OTHER
Source: OTHER
Selection: other
Layout: PAIRED
Construction protocol: mTEC were prepared by digesting thymi with collagenase and dispase, magnetically depleting CD45+ cells, staining with fluorescent antibodies, hashing with TotalSeq antibodies against H2-Kb, and flow sorting mTECs into DMEM/10%FCS. Purified cells were sent to the Broad Institute Genomics Platform, which performed encapsulation and RNA and hash library preparation following 10X Genomics protocols (3' v3.1 chemistry) scRNA-seq