GSM7032534: lean_alpha, biological replicate 5 (RNA-seq); Mus musculu... - SRA

SRX19312489: GSM7032534: lean_alpha, biological replicate 5 (RNA-seq); Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 21.8M spots, 1.6G bases, 392.1Mb downloads

External Id: GSM7032534_r1

Submitted by: Huising lab, Neurobiology, Physiology and Behavior, University of California, Davis

Study: Genome wide assessment of gene expression in alpha and beta cells from lean and obese mice

PRJNA934523 • SRP424210 • All experiments • All runs

show Abstracthide Abstract

We report the assessment of transcriptome by RNA-Seq of FACS purified Ins1-H2bmCherry purified primary mouse beta cells and Gcg-CreERT2 x YFP positive alpha cells of obese (ob/ob) mice compared to lean littermate controls Overall design: Examination of transcriptomes in primary alpha and beta cells in lean vs obob beta cells by RNA-Seq. Islets from 5 lean males, 5 lean females, 2 ob/ob males and 3 ob/ob females between 35-38 weeks of age.

Sample: lean_alpha, biological replicate 5 (RNA-seq)

SAMN33209384 • SRS16711529 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM7032534

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Cells were collected in Trizol post FACS purification and isolated by a chloroform extraction, assisted by phase lock tubes. RNA was precipitated by isopropanol and cleaned up over an RNEasy microcolumn per the Qiagen's taking great care to avoid carry over of ethanol following the column washes. Elution volume was 30 microliter. Indexed sequencing libraries were constructed using the TruSeq RNA sample prep Kit v2 per Illumina instructions.

Runs: 1 run, 21.8M spots, 1.6G bases, 392.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR23371511	21,780,256	1.6G	392.1Mb	2023-09-22

ID:: 26558566

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