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SRX19261141: GSM7020389: Alveolar macrophages from control mouse (sample #2), LPS; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 112.6M spots, 34G bases, 10.4Gb downloads

External Id: GSM7020389_r1
Submitted by: Laboratory Medicine, National Taiwan University Hospital
Study: Alveolar epithelial cells mitigate neutrophilic inflammation in acute lung injury through regulating mitochondrial fatty acid oxidation
show Abstracthide Abstract
Carnitine palmitoyltransferase 1a (CPT1a) is the key regulator of mitochondrial long-chain fatty acid beta-oxidation (LCFAO). However, the functional significance of AT2 cell-specific LCFAO at baseline and during acute lung injury (ALI) is not fully understood. In this study, Murine models of AT2 cell-specific Cpt1a deletion were generated for investigating the role of CPT1a in regulating AT2 cell function and the severity of lipopolysaccharide-induced murine ALI models. Overall design: AT2 cells were isolated from the lungs from mice with AT2 cell-specific Cpt1a deletion (Cpt1a-KO) and the control (Sftpc-CreERt2 +/-) at baseline and 24 hours after intra-tracheal LPS administration. To investigate whether impaired LCFAO in AT2 cells regulates the responses of alveolar macrophages in ALI, we also isolated alveolar macrophages from Cpt1a-KO and control mice at 4 hours after intra-tracheal LPS administration, and alveolar macrophages from control mice at baseline. Alveolar macrophages from 2 mice were pooled to make one sample for trancriptomic analysis.
Sample: Alveolar macrophages from control mouse (sample #2), LPS
SAMN33021193 • SRS16663605 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM7020389
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Cell as input and lysis accroding to manufacturer's instructions of SMART-Seq Stranded Kit (Takara) Libraries were prepared using the SMART-Seq Stranded Kit (Takara)
Runs: 1 run, 112.6M spots, 34G bases, 10.4Gb
Run# of Spots# of BasesSizePublished
SRR23318548112,564,22734G10.4Gb2024-07-05

ID:
26482567

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