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SRX19013825: GSM6929169: HDMB03_PPHLN1_4; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 55.6M spots, 15G bases, 7.1Gb downloads

External Id: GSM6929169_r1
Submitted by: Development and Stem Cell Biology, The Hospital for Sick Children
Study: The OTX2-regulated alternative splicing landscape controls differentiation and reconstitutes the developmental origins of Group 3 medulloblastoma
PRJNA923174 • SRP417142 • All experiments • All runs
show Abstracthide Abstract
RNA sequencing was performed on Group 3 medulloblastoma tumorspheres following PPHLN Morpholino treatment or scramble control. Overall design: A custom splice-blocking morpholino to induce exon skipping was designed for PPHLN1 (ENSG00000134283.13, exon 6, 5'-AGAGTCTAGCTCAAAACTCACCTCT-3'). A concentration of 1 µM in ddH20 was added to HDMB03 single cell suspensions cultured in ultra-low-attachment plates for 5 days prior to RNA extraction.
Sample: HDMB03_PPHLN1_4
SAMN32691255 • SRS16433371 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: GSM6929169
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted from all samples using the Norgen RNA extraction kit (Norgen Biotek), Library constructed with NEB Ultra II directional RNA library prep kit with poly-A selection module
Runs: 1 run, 55.6M spots, 15G bases, 7.1Gb
Run# of Spots# of BasesSizePublished
SRR2306050255,589,70515G7.1Gb2024-05-24

ID:
26171755

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