U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX1888938: GSM2221319: PA1_WT_rep3; Homo sapiens; RIP-Seq
1 ILLUMINA (NextSeq 500) run: 36.8M spots, 2.8G bases, 968.2Mb downloads

Submitted by: NCBI (GEO)
Study: RNA-seq analysis of mRNAs immunoprecipitated by wild-type (WT) or phospho-mimetic (S200D) FLAG-LIN28A in PA1 cells
show Abstracthide Abstract
To explore the effect of LIN28 (S200) phosphorylation on LIN28's association with mRNAs on a transcriptome-wide scale, we performed mRNA-seq on RNAs immunoprecipitated (RIP-seq) by wild-type (WT) or phospho-mimetic (S200D) LIN28 in PA1 cells. Overall design: Examination of mRNAs immunoprecipitated by wild-type and phospho-mimetic (S200D) FLAG-LIN28 (in triplicate)
Sample: PA1_WT_IP_rep3
SAMN05331290 • SRS1532051 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: NextSeq 500
Strategy: RIP-Seq
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: Cells were harvested in cold PBS and immediately lysed in M2 lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100) containing 100 U/ml RNasin (Promega) and 2X protease and phosphatase inhibitors (Pierce). FLAG-tagged LIN28 variants were purified using the anti-FLAG M2 affinity gel following the manufacturer’s specifications (Sigma). Parental PA1 cells (no FLAG) were used as controls for antibody specificity. RNA was isolated from the beads using Trizol (Invitrogen) and RNeasy columns (Qiagen). Purified RNA was subjected to polyA selection using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Libraries were prepared with the NEBNext Ultra RNA Library Prep Kit (NEB).
Experiment attributes:
GEO Accession: GSM2221319
Links:
Runs: 1 run, 36.8M spots, 2.8G bases, 968.2Mb
Run# of Spots# of BasesSizePublished
SRR373316436,774,3692.8G968.2Mb2016-10-31

ID:
2733291

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...