GSM6833403: Clone25; Mus musculus; RNA-Seq - SRA

SRX18700729: GSM6833403: Clone25; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 32M spots, 9.7G bases, 3.6Gb downloads

External Id: GSM6833403_r1

Submitted by: Mouse Cancer Genetics Program, National Cancer Institute

Study: Mismatch repair protein MLH1 suppresses replicative stress in BRCA2 deficient breast tumors

PRJNA912195 • SRP413074 • All experiments • All runs

show Abstracthide Abstract

To investigate the cooperative function KDM4D/NFIB/MLL1 complex in the regulation of Mirin Pretreatment, we established 10T1/2 cell lines in which each target gene has been knocked down by shRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells at two time points. Overall design: Comparative gene expression profiling analysis of RNA-seq data for mESC cells and its KD derivatives (shKDM4D, shNFIB, and shMLL1).

Sample: Clone25

SAMN32232762 • SRS16141805 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM6833403

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Cells were resuspended with QIAzol (QIAGEN, Hilden, Germany) and RNA was extracted (Zymo Direct-zol RNA miniprep kit). RNA libraries were prepared for sequencing using standard Illumina TruSeq Stranded mRNA library protocol.

Runs: 1 run, 32M spots, 9.7G bases, 3.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR22739308	31,997,150	9.7G	3.6Gb	2024-03-04

ID:: 25766036

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity