GSM2198248: Hbb-b1_async_CaptureC_merge; Mus musculus; OTHER - SRA

SRX1850290: GSM2198248: Hbb-b1_async_CaptureC_merge; Mus musculus; OTHER
3 ILLUMINA (NextSeq 500) runs: 39.2M spots, 5.9G bases, 2.2Gb downloads

Submitted by: NCBI (GEO)

Study: A hyperactive transcriptional state marks genome reactivation at the mitosis-G1 transition [CaptureC]

PRJNA325492 • SRP076479 • All experiments • All runs

show Abstracthide Abstract

Capture-C of G1E ER4 cell line in a nocodazole arrest-release experiment anchored at multiple gene promoters to quantify long-range chromatin interactions at the mitosis-G1 transition. Overall design: Nocodazole arrest-release in G1E ER4 cell line under conditions of 13h estradiol treatment (unless otherwise specified as ''unin'', i.e. no estradiol treatment).

Sample: Hbb-b1_async_CaptureC_merge

SAMN05235179 • SRS1508108 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: OTHER

Source: GENOMIC

Selection: other

Layout: PAIRED

Construction protocol: After cell fixation with 1% formaldehyde for 10min and cell sorting as described above, Capture-C was performed with a double-capture procedure (Davies et al. 2016). Chromatin was digested using DpnII. We used biotin labelled DNA oligos to pull down the target restriction fragments. Capture-C libraries were sequenced on Nextseq500 with 2x75 bp paired end sequencing.

Experiment attributes:

GEO Accession: GSM2198248

Links:

NCBI link: NCBI Entrez (gds)

Runs: 3 runs, 39.2M spots, 5.9G bases, 2.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR3674797	14,908,482	2.2G	833.3Mb	2016-06-16
SRR3674798	10,569,483	1.6G	616.1Mb	2016-06-16
SRR3674799	13,698,752	2.1G	799.5Mb	2016-06-16

ID:: 2644199

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