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SRX18413374: GSM6761476: CD8 T cells, GFP control, acute, donor 1, day 14; Homo sapiens; ATAC-seq
1 ILLUMINA (NextSeq 2000) run: 40.7M spots, 4.1G bases, 1.2Gb downloads

External Id: GSM6761476_r1
Submitted by: Charles Gersbach, Biomedical Engineering, Duke University
Study: CRISPR-based epigenome editing screens identify transcriptional and epigenetic regulators of human CD8 T cell function [ATAC-seq]
show Abstracthide Abstract
Human CD8+ T cells were transduced with lentivirus encoding for HER2-CAR-2A-GFP or HER2-CAR-2A-BATF3. T cells were either only stimulated once after thawing (acute stimulation) or repeatedly stimulated with HER2+ SKBR3 cells at a 1:2 effector to target (E:T) ratio every 3 days (chronic stimulation). Overall design: Comparative chromatin accessibility analysis of ATAC-seq data of acutely (n = 3) and chronically (n = 2) stimulated CD8+ T cells with or without BATF3 overexpression.
Sample: CD8 T cells, GFP control, acute, donor 1, day 14
SAMN31927055 • SRS15896341 • All experiments • All runs
Organism: Homo sapiens
Library:
Name: GSM6761476
Instrument: NextSeq 2000
Strategy: ATAC-seq
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: 50,000 transduced CD8 T cells were sorted for ATAC-seq. ATAC-seq libraries were prepared according to the Omni ATAC-seq protocol.
Runs: 1 run, 40.7M spots, 4.1G bases, 1.2Gb
Run# of Spots# of BasesSizePublished
SRR2244446140,664,7174.1G1.2Gb2023-05-24

ID:
25437951

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