GSM2194815: ACGI428 29493; Homo sapiens; RNA-Seq - SRA

SRX1832665: GSM2194815: ACGI428 29493; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 2.4M spots, 238.2M bases, 104.8Mb downloads

Submitted by: NCBI (GEO)

Study: Single cell RNA-seq of human pancreatic endocrine cells from Juvenile, adult control and type 2 diabetic donors.

PRJNA325005 • SRP076307 • All experiments • All runs

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We successfully sequenced and annotated more than 400 cells from child, adult control, type 1 diabetes and type 2 diabetes donors. We detect donor-type specific transcript variation. We also report that cells from child donors have less defined gene signature. Cells from type 2 diabetes donors resemble juvenile cells in gene expression. Overall design: Cells from three adult controls (56, 74, 92), one donor with type 1 diabetes (91), two donors with type 2 diabetes (75, 143), and two child donors (40, 72) were sequenced. Numbers in parathesis indicates number of cells sequenced.

Sample: ACGI428 29493

SAMN05217167 • SRS1493742 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Islets were dissociated to single cell by 0.05% Trypsin, 9 mins at 37oC. Cells (250-500 cells/μl) were subsequently loaded on Fluidigm C1 IFC with either 5-10 μM or 10-17 μM capture size. Smarter-seq V2 kit was utilized for library construction per manufacturer's protocol to generate cDNA. Prepration of Illumina libraries from cDNA was done with the NexteraXT kit per Fluidigm’s instructions.

Experiment attributes:

GEO Accession: GSM2194815

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 2.4M spots, 238.2M bases, 104.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR3650970	2,382,140	238.2M	104.8Mb	2016-07-05

ID:: 2617198

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