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SRX18306221: GSM6739158: H3K4me1 exc rep2; Mus musculus; OTHER
1 ILLUMINA (Illumina NovaSeq 6000) run: 6.5M spots, 2G bases, 894.1Mb downloads

External Id: GSM6739158_r1
Submitted by: Epigenomics and Computational Biology Laboratory, Department of Biomedical Sciences and Pathobiology, Virginia Tech
Study: Histone modifications mediate transcription factor regulation and contribute to neuronal subtype identity
PRJNA903145 • SRP408603 • All experiments • All runs
show Abstracthide Abstract
To investigate the cell-type specific histone modification and transcription factor bindings, we performed cut and run of four histone modifications and the transcription factor EGR1 in excitatory and inhibitory neurons in adult mouse brain. Overall design: CUT&RUN
Sample: H3K4me1 exc rep2
SAMN31785163 • SRS15796875 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM6739158
Instrument: Illumina NovaSeq 6000
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: Nuclei were isolated from adult mouse brain tissue and subjected to FACS-sorting. The FACS-sorted nuclei were used for Cut&Run with the CUTANA CUT&RUN Kit. The Cut&Run enriched DNA fragments were used for DNA library construction. Cut&Run libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer's instructions.
Runs: 1 run, 6.5M spots, 2G bases, 894.1Mb
Run# of Spots# of BasesSizePublished
SRR223333586,523,1692G894.1Mb2023-11-18

ID:
25316729

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