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SRX1791122: GSM2174459: 2Snf5Snf2IP (ChIP-Seq); Saccharomyces cerevisiae; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 25.3M spots, 1.3G bases, 824.6Mb downloads

Submitted by: NCBI (GEO)
Study: Composition and Function of Mutant Swi/Snf Complexes
show Abstracthide Abstract
Loss of Swi/Snf subunits regulate transcription. Overall design: RNA-seq and ChIP-seq for Snf2 was done in Swi/Snf subunit deletion mutant yeast strains
Sample: 2Snf5Snf2IP (ChIP-Seq)
SAMN05162314 • SRS1461366 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Yeast whole cell extract were sonicated DNA-protein complexes were isolated with Snf2-C'antibody (kind gift from Joseph Reese Pennsylvania State University: antibody raised in Reese lab) . ChiP-seq, Mnase-seq, RNA-seq libraries were prepared using KAPA High Throughput Library Preparation Kit, Roche.
Experiment attributes:
GEO Accession: GSM2174459
Links:
Runs: 1 run, 25.3M spots, 1.3G bases, 824.6Mb
Run# of Spots# of BasesSizePublished
SRR356799725,310,1831.3G824.6Mb2017-02-17

ID:
2563538

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