U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX17823952: GSM6619531: H3K27ac_Young_Rep2; Mus musculus; ChIP-Seq
1 ILLUMINA (NextSeq 500) run: 57.5M spots, 4.3G bases, 1.5Gb downloads

External Id: GSM6619531_r1
Submitted by: Shantou University Medical College
Study: Transcriptional dysregulation impairs efficacy of neural stem cell activation during aging [ChIP-seq]
show Abstracthide Abstract
To get insight into the histone modification in the transcriptional dysregulation of NSC aging, we profiled the principal regulatory histone marks at the promoters and enhancers of age-dependent genes using ChIP-sequencing. Overall design: Primary NSCs were isolated from the subventricular zone of the lateral ventricles (SVZ) in the brains of young and aged mice, respectively. The NSCs were maintained in the initial proliferation medium and used in ChIP-sequencing analysis.
Sample: H3K27ac_Young_Rep2
SAMN31190291 • SRS15347877 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM6619531
Instrument: NextSeq 500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: NSCs were fixed with 1% formaldehyde for 10 min and quenched with glycine solution for 20 min at room temperature. The fixed NSCs were lysed in ChIP lysis buffer on ice. Chromatin was released during cell lysis and sonicated using a E220 focused-ultrasonicator (Covaris). ChIP-Seq libraries were prepared based on the protocol of KAPA HyperPrep Kit (Roche) complemented with the NEXTflex DNA Barcodes (Bioo Scientific).
Runs: 1 run, 57.5M spots, 4.3G bases, 1.5Gb
Run# of Spots# of BasesSizePublished
SRR2183486157,543,2794.3G1.5Gb2023-11-02

ID:
24750111

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...