GSM6597352: Bjab cell line, stable transfection short version of TACI... - SRA

SRX17676805: GSM6597352: Bjab cell line, stable transfection short version of TACI, replicate 1; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 52.9M spots, 15.9G bases, 4.8Gb downloads

External Id: GSM6597352_r1

Submitted by: Medicine, Icahn School of Medicine at Mount Sinai

Study: Endogenous B cell TACI-Short-APRIL signaling

PRJNA883412 • SRP398743 • All experiments • All runs

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Here we show that TACI-S increases its expression with maturation and colocalizes in the intracellular compartment with the ligand APRIL. APRIL downregulation in human B cells, eliminated class switch. These studies suggest the importance of intracellular APRIL together with TACI-S in maintaining humoral immunity. Overall design: TACI isoforms, long and short, are coexpressed in human B cells.To analyze independently the functional effects of TACI isoforms, we transduced each one in human BJAB B cell line and analyzed their transcriptional program in resting condition

Sample: Bjab cell line, stable transfection short version of TACI, replicate 1

SAMN30965199 • SRS15208827 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM6597352

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA from the sorted cells was isolated using the RNeasy Plus Micro kit (QIAGEN). Quality control was performed by bioanalyzer (Agilent), and RNA samples with an RNA integrity number (RIN) > 8 were processed for library preparation using Nextera XT DNA (Illumina) library preparation kits

Runs: 1 run, 52.9M spots, 15.9G bases, 4.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR21678532	52,921,007	15.9G	4.8Gb	2023-09-22

ID:: 24576615

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