SRA

Background: Prior analyses of mRNA expression in intestinal tissue as related to HIV cohorts has been on whole biopsies. More targeted isolation and enrichment of intestinal epithelial cells is needed to identify pathways specifically altered in the epithelium that may not be picked up at a global tissue scale. Results: Analysis of intestinal epithelial cells isolated from primary human tissues taken from HIV-infected cohorts as well as uninfected controls. Results demonstrate key genes differentially expressed in HIV in the absence or presence of ART-therapy. Overall design: Cross-sectional sampling and comparison of epithelial isolates from 9 uninfected, 6 viremic untreated, and 19 HIV-infected ART-treated patients. Whole biopsy RNA from 5 uninfected, 6 viremic untreated, and 5 HIV-infected ART-treated patients.