GSM6529490: Roots, protoplasted, rep 1; Solanum lycopersicum; RNA-Seq - SRA

SRX17381385: GSM6529490: Roots, protoplasted, rep 1; Solanum lycopersicum; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 9.4M spots, 2.8G bases, 1.1Gb downloads

External Id: GSM6529490_r1

Submitted by: Plant Biology and Genome Center, UC Davis

Study: Protoplast data: suberized exodermis is required for tomato drought tolerance

PRJNA875392 • SRP395059 • All experiments • All runs

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This experiment aimed to identify protoplast-induced genes in tomato seedling, for future use in single cell datasets. Overall design: Comparative gene expression profiling analysis of RNA-seq data for tomato seedling roots (protoplasted vs non-protoplasted).

Sample: Roots, protoplasted, rep 1

SAMN30615195 • SRS14940561 • All experiments • All runs

Organism: Solanum lycopersicum

Library:

Name: GSM6529490

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted using the Direct-zol RNA Miniprep kit (ZYMO). Bulk RNAseq libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen). Barcoded libraries were then pooled together, and PE 150-bp reads were sequenced on the NovaSeq 6000 instrument (Illumina) at the UC Davis DNA Technologies Core.

Runs: 1 run, 9.4M spots, 2.8G bases, 1.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR21375716	9,436,891	2.8G	1.1Gb	2023-10-01

ID:: 24203928

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