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SRX15457919: GSM6195545: P19, WT, BMS641+BMS961, Day 4; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 32.8M spots, 9.4G bases, 2.8Gb downloads

External Id: GSM6195545_r1
Submitted by: Genoscope
Study: Synergistic activation of RARb and RARg-driven programs restores cell-types specialization during stem cell differentiation
show Abstracthide Abstract
How cells respond to different external cues to develop along defined cell lineages composing complex tissues is a major question in systems biology. In this study, we studied the role of synthetic agonists targeting specific Retinoic acid receptors (RARs) on activating major gene regulatory wires driving cell specialization during nervous tissue formation issued from P19 stem cells. Specifically, we have revealed that the synergistic activation of the RAR? and RAR? nuclear receptors by their cognate ligands (BMS641 or BMS961) induce neuronal maturation, inlcuding specialized neuronal subtypes as well as to astrocytes and oligodendrocyte precursors. With the help of Rar-null mutant lines, combined with the use of RAR-specific agonists, global transcriptome mapping and an in-silico modeling of transcription regulatory signalling propagation, we have highlighted major gene programs essential for optimal neuronal cell specialization. Overall, this study provides a systems biology view of the gene programs behind the previously observed redundancy between RAR receptors, paving the way for their potential use for directing cell specialization during nervous tissue formation. Overall design: Comparative gene expression profiling analysis of RNA-seq data for WT P19 or rar-null mutant EC cells treated with ATRA, BMS753, BMS641, BMS961 or a combination of BMS641 & BMS961 .
Sample: P19, WT, BMS641+BMS961, Day 4
SAMN28660197 • SRS13178697 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM6195545
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted from P19 cells treated with either ATRA or RAR-specific agonists, using the TRIzol RNA isolation reagent (ThermoFisher Scientific ref: 15596026). RNA-sequencing libraries were produced with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (E7770). poly-T captured mRNA-seq
Runs: 1 run, 32.8M spots, 9.4G bases, 2.8Gb
Run# of Spots# of BasesSizePublished
SRR1940335232,803,4459.4G2.8Gb2022-11-10

ID:
22014878

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