Name: GSM6071122
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Cells were harvested and resuspended in 1ml of QIAzol Lysis reagent (Qiagen, Cat. No.79306). Total RNA was then purified with RNeasy Plus Mini kit (Qiagen, cat. No. 74134) with minimal modifications to manufacturer's instructions. DNase treatment (RNase free DNase set, Qiagen, Cat. No. 79254) was performed to remove any residual DNA. RNA quality and concentration were checked using a NanodropTM 2000 spectrophotometer (ThermoFisher). Total RNA-seq library was prepared with CORALL Total RNA library prep with RiboCop rRNA for Human/Mouse/Rat depletion kit (Lexogen GmbH, Vienna, Austria) following manufacturer's instructions (library type: fr-secondstrand) by IGA Technology service (Italy). Final libraries were checked with both Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and Agilent Bioanalyzer DNA assay or Caliper (PerkinElmer, Waltham, MA).