GSM6071084: siEHMT1-rep 1; Homo sapiens; RNA-Seq - SRA

SRX15038588: GSM6071084: siEHMT1-rep 1; Homo sapiens; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2500) runs: 18.7M spots, 947M bases, 396.2Mb downloads

External Id: GSM6071084_r1

Submitted by: University of Massachusetts Boston

Study: Lysine methylation of EHMT1/GLP as a molecular switch to reprogram transcription networks in prostate cancer [RNA-seq]

PRJNA832968 • SRP372663 • All experiments • All runs

show Abstracthide Abstract

Our studies provide novel molecular insights into the activity and function of EHMT1 during PCa progression and suggest a novel therapeutic strategy to treat CRPC with EHMT1 inhibitors. Overall design: LNCaP-tet-EHMT1 cells were treated with/out 0.25ug/mL doxycycline for two days. LNCaP cells were treated with siNTC, siEHMT1 at 20nM for two days. All RNA-seq samples contain replications.

Sample: siEHMT1-rep 1

SAMN27926440 • SRS12785440 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM6071084

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted with Rneasy Mini Kit (QIAGEN) following manufacturer's protocol. RNA-seq libraries of both LNCaP-tet-EHMT1 and LNCaP cells were performed by using TruSeq® Strnd Total RNA LT (Illumina).

Runs: 2 runs, 18.7M spots, 947M bases, 396.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR18961491	9,270,570	468.5M	196.1Mb	2023-09-14
SRR18961492	9,470,307	478.5M	200Mb	2023-09-14

ID:: 21510924

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