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SRX14967153: GSM6057992: small RNA-seq for chimpanzee iPS cells #2; Pan troglodytes; ncRNA-Seq
1 ILLUMINA (Illumina HiSeq 1500) run: 11.1M spots, 563.4M bases, 169.4Mb downloads

External Id: GSM6057992_r1
Submitted by: Laboratory of Genome and Epigenome Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University
Study: TE-derived small RNA analysis of human and chimpanzee iPS cells
show Abstracthide Abstract
Small RNA-seq were conducted for iPS cells of human-1 (409-B2/HPS0076), human-2 (Nips-B2/HPS0223), chimpanzee-1 (kiku/0138F-1), and chimpanzee-2 (mari/0274F-2). The human samples were mapped to the human genome (hg38) and the chimpanzee samples were mapped to the chimpanzee genome (panTro5). The mapped reads for individual TE copies (in the repeatmasker tables downloaded from UCSC genome browser) were counted, and those for the same subfamily were summed up. The counts were normalized by RPM (reads per million genome-mapped reads). Overall design: Two human and two chimpanzee iPS cell lines (all are females and 36 to 43 years old) were cultured in the same condition. RNAs were then extracted, and small RNA sequencing libraies were made for HiSeq sequencing in the 50-bp single-end mode.
Sample: small RNA-seq for chimpanzee iPS cells #2
SAMN27738462 • SRS12717279 • All experiments • All runs
Organism: Pan troglodytes
Library:
Name: GSM6057992
Instrument: Illumina HiSeq 1500
Strategy: ncRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Total cellular RNAs were isolated from ~1×106 cells by using Isogen (TOYOBO) and Direct-ZolTM RNA (ZYMO RESEARCH) according to manufactures' instructions. After quality check by Agilent 2100 Bioanalyzer (Agilent Technologies), small RNA-seq libraries were prepared from approximately 0.5 μg of total RNA using NEBNext small RNA library kit (New England Biolabs). After library amplification by PCR, products were run on 6% polyacrylamide gel electrophoresis, and a gel region corresponding to DNA sizes with 15 to 40 bp insertion were cut for DNA extraction.
Runs: 1 run, 11.1M spots, 563.4M bases, 169.4Mb
Run# of Spots# of BasesSizePublished
SRR1888855911,078,297563.4M169.4Mb2022-10-26

ID:
21420745

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