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SRX1452774: GSM1954942: WT_ESC_H3K4me1_IP_R2; Mus musculus; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 18M spots, 1.8G bases, 1.1Gb downloads

Submitted by: NCBI (GEO)
Study: Local compartment changes and regulatory landscape alterations in histone H1-depleted cells
show Abstracthide Abstract
Linker histone H1 is a core chromatin component that binds to nucleosome core particles and the linker DNA between nucleosomes. It has been implicated in chromatin compaction and gene regulation and is anticipated to play a role in higher-order genome structure. We find that depletion of histone H1 changes the epigenetic signature of thousands of potential regulatory sites across the genome. Many of them show cooperative loss or gain of multiple chromatin marks. Epigenetic alterations cluster to gene-dense topologically associated domains (TADs) that already showed a high density of corresponding chromatin features. Genome organization at the three-dimensional level is largely intact, but we find changes in the structural segmentation of chromosomes specifically for the epigenetically most modified TADs. Overall design: We have used a combination of genome-wide approaches including DNA methylation, histone modification and DNaseI hypersensitivity profiling as well as Hi-C to investigate the impact of reduced cellular levels of histone H1 in embryonic stem cells on chromatin folding and function.
Sample: WT_ESC_H3K4me1_IP_R2
SAMN04296665 • SRS1180474 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: TruSeq DNA sample preparation kit (Illumina).
Experiment attributes:
GEO Accession: GSM1954942
Links:
Runs: 1 run, 18M spots, 1.8G bases, 1.1Gb
Run# of Spots# of BasesSizePublished
SRR296159218,006,0981.8G1.1Gb2015-11-30

ID:
2052185

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