RIP-H9-MSI1V272-Flag - SRA

SRX14271998: RIP-H9-MSI1V272-Flag
1 ILLUMINA (Illumina NovaSeq 6000) run: 19.9M spots, 6G bases, 2.1Gb downloads

Design: RNA immunoprecipitation was performed with reversible cross-linking of the cells, as previously described (Niranjanakumari et al., 2002). The RNA was extracted from these samples using Trizol reagent (Invitrogen) .RNA quality was determined. High-quality RNA sample is used to construct sequencing library. RNA-seq transcriptome libraries were prepared following TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA). Messenger RNA was isolated with poly-A selection by oligo(dT) beads and fragmented. cDNA synthesis, end repair, A-base addition and ligation of the Illumina-indexed adaptors were performed according to Illumina protocol. Libraries were then size selected for cDNA target fragments on 2% Low Range Ultra Agarose (Sigma-Aldrich) followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles. After quantification, paired-end libraries were sequenced by Illumina NovaSeq 6000 sequencing

Submitted by: Tongji University

Study: Musashi1 and short C-terminal MSI1-C variants regulate embryonic stem cell pluripotency

PRJNA808680 • SRP360646 • All experiments • All runs

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To understand the function of MSI1 in pluripotent stem cells, RNA-seq assays were performed on mouse embryonic stem cells R1, MSI1 knockout cell line R1-C5, human embryonic stem cells H9, RRM knockout cell line H9-C8, MSI1 full-length overexpression cell line H9-MSI1OE, MSI1C variant overexpression cell line H9-MSI1 (138-362) OE , H9-MSI1(272-362)OE. RNA bound by MSI1 in R1 and H9, and MSI1C variants MSI1 (138-362), MSI1(272-362) were detected using RIP-seq.

Sample:

SAMN26209681 • SRS12090650 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: RIP-H9-MSI1V272-Flag

Instrument: Illumina NovaSeq 6000

Strategy: RIP-Seq

Source: TRANSCRIPTOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 19.9M spots, 6G bases, 2.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR18121575	19,872,092	6G	2.1Gb	2024-03-14

ID:: 20229149

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