GSM5914501: D 92 high concentrate feeding replicate 4; Bos taurus; mi... - SRA

SRX14266539: GSM5914501: D 92 high concentrate feeding replicate 4; Bos taurus; miRNA-Seq
1 ION_TORRENT (Ion Torrent S5) run: 7M spots, 225.5M bases, 171.6Mb downloads

External Id: GSM5914501_r1

Submitted by: Animal and Veterinary Science, Clemson University

Study: Identification of microRNA transcriptome involved in bovine intramuscular fat deposition

PRJNA809735 • SRP361179 • All experiments • All runs

show Abstracthide Abstract

Longissimus muscle samples were collected from angus-cross steers while being fed a high concentrate diet. Sample were collected on d 0, 92, and 124 of high concentrate feeding. Muscle samples were examined to evaluate the miRNA involved in bovine intramusclular fat deposition. Overall design: The study is comprised of 12 samples, there are 3 groups (0, 92, and 124 d of high concentrate feeding) with 4 replicates each.

Sample: D 92 high concentrate feeding replicate 4

SAMN26200310 • SRS12085575 • All experiments • All runs

Organism: Bos taurus

Library:

Name: GSM5914501

Instrument: Ion Torrent S5

Strategy: miRNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: Extraction of total RNA was extracted with Trizol according to manufactures specifications. Qantificatioon and qualification were checked by NanoPhotometer spectrophotometer and Agilent Bioanalyzer 2100 system. cDNA libraries were constructed using Ion Total RNA-Seq Kit v2 according to the manufacturer.

Runs: 1 run, 7M spots, 225.5M bases, 171.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR18116009	7,023,155	225.5M	171.6Mb	2022-02-26

ID:: 20213278

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