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SRX13755762: GSM5814048: P0_ERa_female_E2_CR_1; Mus musculus; OTHER
1 ILLUMINA (NextSeq 500) run: 39.8M spots, 6.1G bases, 2Gb downloads

Submitted by: NCBI (GEO)
Study: Gene regulation by gonadal hormone receptors defines brain sex differences-[CUT&RUN]
show Abstracthide Abstract
Estradiol establishes neural sex differences in many vertebrates and modulates mood, behavior, and energy balance in adulthood. In the canonical pathway, estradiol exerts its effects through the transcription factor estrogen receptor a (ERa). While ERa has been extensively characterized in breast cancer, the neuronal targets of ERa, and their involvement in brain sex differences, remain largely unknown. Here we generate a comprehensive map of genomic ERa-binding sites within a sexually dimorphic neural circuit that mediates social behaviors. We conclude that ERa orchestrates sexual differentiation of the mouse brain through two mechanisms: establishing two male-biased neuron types and activating a sustained male-biased gene expression program. Collectively, our findings reveal that sex differences in gene expression are defined by hormonal activation of neuronal steroid receptors. The molecular targets we identify may underlie the effects of estradiol on brain development, behavior, and disease. Overall design: Genomic response to estradiol in mouse brain
Sample: P0_ERa_female_E2_CR_1
SAMN24900542 • SRS11633109 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: Tissue (4-5 animals pooled per replicate) was homogenized 15x with a loose pestle in a glass homogenizer containing Homogenization Medium (250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 20 mM Tricine-KOH, 1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, 1X Roche EDTA-free protease inhibitor cocktail, pH 7.8). 0.3% IGEPAL CA-630 was added, and the tissue was further dounced 5x with a tight pestle. After douncing, the homogenate was filtered through a 40 µm strainer.The nuclei were diluted 2:1 with cold, supplemented homogenization buffer, and 2mM EDTA was added. The sample was immediately sorted using the Sony SH800S Cell Sorter (purity mode) with a 100-μm sorting chip. 150,000 GFP+ nuclei were collected into CUT&RUN wash buffer. GFP- events were collected into CUT&RUN wash buffer, and 150,000 nuclei were subsequently counted on the Countess II FL Automated Cell Counter for ERα- and IgG negative control CUT&RUN. Takara SMARTer ThruPlex DNA-seq Kit
Experiment attributes:
GEO Accession: GSM5814048
Links:
Runs: 1 run, 39.8M spots, 6.1G bases, 2Gb
Run# of Spots# of BasesSizePublished
SRR1758688739,814,5926.1G2Gb2022-04-21

ID:
19139914

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