GSM5777348: an4_4h; [Haemophilus] ducreyi; RNA-Seq - SRA

SRX13658747: GSM5777348: an4_4h; [Haemophilus] ducreyi; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 36.3M spots, 7.3G bases, 2.4Gb downloads

External Id: GSM5777348_r1

Submitted by: Department of Microbiology & Immunology, Indiana University

Study: Genes differentially expressed by Haemophilus ducreyi during anaerobic growth significantly overlap with those differentially expressed during experimental infection of human volunteers

PRJNA795492 • SRP353998 • All experiments • All runs

show Abstracthide Abstract

The transcriptional response of Haemophilus ducreyi to anaerobiosis was determined over the course of 18 h. Cells were collected from aerobic and anaerobic cultures at 4, 8, or 18 hours . Overall design: Overnight aerobic cultures of H. ducreyi were subcultured into GC broth plus hemin, FBS, and IsoVitalex that contained either the commercial product Oxyrase or the buffer components of Oxyrase to yield anaerobic and aerobic growth conditions, respectively, and grown for up to 18 h.

Sample: an4_4h

SAMN24725786 • SRS11544581 • All experiments • All runs

Organism: [Haemophilus] ducreyi

Library:

Name: GSM5777348

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: 5 mL of culture was pelleted, media was removed and the pellet was resuspended in 1 mL of Trizol. Samples were stored at -80 until all samples were collected. RNA was extracted using the Zymo Directzol kit with both on-column DNase digestion as well as a subsequent DNase digestion of the eluate with TurboDNase. Ribosomal RNA was removed with Qiagen FastSelect for bacteria. Libraries were prepared with a Roche KAPA kit.

Runs: 1 run, 36.3M spots, 7.3G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR17488525	36,250,109	7.3G	2.4Gb	2022-03-28

ID:: 18994715

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