show Abstracthide AbstractWe challenge bristol strain (wild-type), metl-9 KO strain (short as KO, has a 101bp insertion, leads to a truncated protein of 258aa) and metl-9 catalytic-activity mutated strain (short as mut, has N172K, D274G mutations in full-length protein) with P.aeruginosa (P.A14), and observe a discrepant transcriptome pattern between wild-type and KO/mut strains. Plenty of innate immune response genes show different expression patterns upon P.A14 infection between the wild-type strain and KO/mut strain. It indicates the important role of metl-9 and 6mA in worm innate immune response modulation. Overall design: RNA-seq of wild-type, KO or mut strain upon P.A14 infection, or normal condition (OP50).