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SRX13328057: GSM5719324: alveolar macrophage, NC-2, ATAC-seq; Sus scrofa; ATAC-seq
1 ILLUMINA (Illumina HiSeq 4000) run: 38.5M spots, 11.6G bases, 4Gb downloads

Submitted by: NCBI (GEO)
Study: Transcriptomic and chromatin accessibility analyses of porcine alveolar macrophages exposed to Fumonisin B1
show Abstracthide Abstract
Fumonisin B1 (FB1) is a mycotoxin that poses a great threat to agricultural production and human and animal health. However, the molecular mechanism underlying the cytotoxic effect of FB1 to mammals has not been systematically elucidated. Here, we utilized the porcine alveolar macrophage cell line 3D4/21 as model, and applied RNA sequencing (RNA-seq) to analyze the genome-wide transcriptional alterations of mRNA, lncRNA, circRNA and miRNA before and after exposure to FB1 in six samples. To further reveal the underlying regulatory mechanism, we applied Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq) to determine the genome-wide chromatin accessibility alterations in response to FB1-induced cytotoxicity. We anticipate that this dataset will serve as valuable resource for clarifying the transcriptional and regulatory mechanism underlying the cytotoxic effect of FB1, and facilitate the identification of the key genes and signaling pathways contributing to cellular response to FB1 exposure. Overall design: We analyzed the transcriptional alterations of mRNA, lncRNA, circRNA and miRNA in porcine alveolar macrophages before and after exposure to FB1 using RNA-Seq. Using ATAC-seq technology, we determined changes in genome-wide chromatin accessibility in porcine alveolar macrophages in response to FB1-induced cytotoxicity.
Sample: alveolar macrophage, NC-2, ATAC-seq
SAMN23705695 • SRS11234562 • All experiments • All runs
Organism: Sus scrofa
Library:
Instrument: Illumina HiSeq 4000
Strategy: ATAC-seq
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: The cell samples were collected and the nuclei were extracted, and the transposable mixture containing Tn5 transposase was added to the nuclear suspension for transposable reaction. Tn5 transposase entered the nucleus and preferentially cleaved exposed DNA in the open region of chromatin, while ligating specific sequencing adaptor. The DNA fragments ligated with adaptors were amplified by PCR, and the amplified PCR products were purified with the Qiagen MinElute kit and sequenced by Gene Denovo Biotechnology Company (Guangzhou, China).
Experiment attributes:
GEO Accession: GSM5719324
Links:
Runs: 1 run, 38.5M spots, 11.6G bases, 4Gb
Run# of Spots# of BasesSizePublished
SRR1714401138,502,38411.6G4Gb2022-11-10

ID:
18284252

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