U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX13225283: GSM5703498: CKO; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 298.7M spots, 58G bases, 17Gb downloads

Submitted by: NCBI (GEO)
Study: The role of RPA1 in T cell
PRJNA783417 • SRP347802 • All experiments • All runs
show Abstracthide Abstract
As a single strand DNA binding protein, RPA1 participates various cellular processes including DNA replication and DNA repair.However, the role of RPA1 in T cell is largely unknown. We generate a mice model in which RPA1 was specifically deleted in T cell. Through single cell RNA sequencing, we reveal the immune landscape in RPA1 conditional knockout mice. Overall design: Study of immune cells in lymph nodes from WT or RPA1 conditional knockout mice using 10x scRNA-seq technologies.
Sample: CKO
SAMN23429412 • SRS11150662 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Single cells were mixed with Gel Beads, Partitioning Oil and glycerol, using for preparation of GEMs with ChromiumTM Next GEM Chip G Single Cell Kit. The GEMs were subjected to RT-PCR, and the products were purified with Dynabeads, followed by cDNA amplification. RNA libraries were prepared with ChromiumTM Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 according to the manufacturer's protocol.
Experiment attributes:
GEO Accession: GSM5703498
Links:
Runs: 1 run, 298.7M spots, 58G bases, 17Gb
Run# of Spots# of BasesSizePublished
SRR17035396298,713,40858G17Gb2024-06-16

ID:
18088627

Supplemental Content

Related information

Search details

See more...

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...