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SRX1309152: GSM1902813: Med20 C; Schizosaccharomyces pombe; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 13.4M spots, 2.4G bases, 1.5Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Comparison of RNA profiling between wild type, med20?, rrp6? and med20?/rrp6? in Schizosaccharomyces pombe
show Abstracthide Abstract
Mediator is a co-regulator of RNA polymerase II (Pol II), transducing signals from regulatory elements and transcription factors to the general transcription machinery at the promoter. We here demonstrate that Med20 influences ribosomal protein expression in fission yeast. In addition, loss of Med20 leads to an accumulation of aberrant readthrough tRNA transcripts. The aberrant transcripts are polyadenylated and targeted for degradation by the exosome.  Similarly, other specialized RNA molecules, such as snRNA, snoRNA and rRNA are also accumulated in the absence of Med20. We suggest that fission yeast Mediator takes part in a regulatory pathway of Pol III transcripts. Overall design: RNA-sequencing of wt, med20?, rrp6?, med20?/rrp6? strains using wt as control. Each strain was sequenced using three biological replicas. The results were analyzed for differential expression.
Sample: Med20 C
SAMN04147862 • SRS1102362 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Hot Phenol RNA extraction, Qiagen RNA-easy clean up Poly-A enrichment using oligo(dT) beads. cDNA synthesis using Invitrogen Supercript III. Incorporation of dUTP on second strand. Standard Illumina library preparation protocol.
Experiment attributes:
GEO Accession: GSM1902813
Links:
Runs: 1 run, 13.4M spots, 2.4G bases, 1.5Gb
Run# of Spots# of BasesSizePublished
SRR256779213,355,9642.4G1.5Gb2016-01-04

ID:
1867601

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