Instrument: Illumina Genome Analyzer IIx
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: total RNA was extracted from each cell line using Trizol RNA extraction reagent (Life Technologies), according to manufacturer’s recommendations and 75 µg was used in two rounds of polyA selection to purify the mRNA. The mRNA was reverse transcribed into cDNA and made into dsDNA using Just cDNA Double Stranded cDNA Synthesis Kit (Stratagene). The double stranded products were fragmented using Covaris S2 System, size selected using Ampure XP beads (Beckman Coulter), then sequencing libraries prepared before single end deep sequencing was carried out with an Illumina GA IIx to obtain 80bp reads.