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SRX1295726: GSM1898302: shH1.0-1 DOX H3K27me3 biological replicate 1; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 42M spots, 8.5G bases, 2.5Gb downloads

Submitted by: NCBI (GEO)
Study: The linker histone H1.0 generates epigenetic and functional intratumor heterogeneity [ChIP-Seq]
show Abstracthide Abstract
Identification of enrichment for H3K27ac and H3K27me3 in in vitro generated CSCs, following the knockdown of the histone linker H1.0 Overall design: ChIP-seq analysis of biological replicates from SSEA1+ in vitro transformed human skin fibroblasts in the presence or absence of H1.0
Sample: shH1.0-1 DOX H3K27me3 biological replicate 1
SAMN04122768 • SRS1094511 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Construction protocol: ChIP was performed as detailed by Sailaja et al PNAS 109:E3687-3695 2012 Paired end libraries were constructed using standard Illumina protocols, with some modifications. Agencourt AMPure XP (Beckman Coulter) at 0.8x ratio were used to size select out adapter dimers. The Illumina Phusion enzyme was replaced by Kapa HiFi HotStart ready mix (Kapa Biosystems). An Invitrogen SizeSelect R-gel system (Life Technologies) was used to size select following PCR amplification. Sequenced was performed on an Illumina HiSeq 2500 at The Crick Institute, London.
Experiment attributes:
GEO Accession: GSM1898302
Links:
Runs: 1 run, 42M spots, 8.5G bases, 2.5Gb
Run# of Spots# of BasesSizePublished
SRR253713642,004,2888.5G2.5Gb2016-09-30

ID:
1851806

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