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SRX1292527: GSM1896290: Het (Q111) 2 month Female Hippocampus mRNA rep4; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 53.3M spots, 5.4G bases, 1.9Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Transcriptome profiling in knock-in mouse models of Huntington''s disease [Hippocampus_mRNA]
show Abstracthide Abstract
Huntington''s disease (HD) is an autosomal dominant neurodegenerative disorder that is characterized by motor, cognitive, and psychiatric alterations. The mutation responsible for this disease is an abnormally expanded and unstable CAG repeat within the coding region of the gene encoding huntingtin (Htt). Knock-in mouse models of HD with human exon 1 containing expanded CAG repeats inserted in the murine huntingtin gene (Hdh) provide a genetic reconstruction of the human causative mutation within the mouse model. The goal of this study is RNA expression profiling by RNA sequencing (RNA-seq) in 2, 6, and 10 month old knock-in mice with CAG lengths of 20, 80, 92, 111, 140, 175 along with littermate control wild-type animals Overall design: mRNA expression profiles were obtained via RNA-seq analysis performed on tissue samples from the hippocampus of 2, 6, and 10 month old knock-in mice with CAG lengths of 20, 80, 92, 111, 140, 175 along with littermate control wild-type animals.
Sample: Het (Q111) 2 month Female Hippocampus mRNA rep4
SAMN04116299 • SRS1092027 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Tissue specimens were removed, flash frozen on dry ice, and total RNA was harvested using the Qiagen miRNeasy kit. RNA quality and integrity were monitored via Agilent Bioanalyzer. A minimum of 3.75 ug of total RNA were used to collect enriched RNA subfractions for library construction. RNA libraries were prepared for sequencing using standard Illumina protocols.
Experiment attributes:
GEO Accession: GSM1896290
Links:
Runs: 1 run, 53.3M spots, 5.4G bases, 1.9Gb
Run# of Spots# of BasesSizePublished
SRR253139953,305,6315.4G1.9Gb2015-11-03

ID:
1847650

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