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SRX12735267: GSM5651033: 4T1-MCF7 mixed single-omics sample RNA; Homo sapiens; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 96.3M spots, 28.9G bases, 14.7Gb downloads

Submitted by: NCBI (GEO)
Study: Decoding Expression Dynamics of Protein and Transcriptome at the Single Cell Level through Multi-Omics Sequencing in Paired Picoliter Chambers
show Abstracthide Abstract
Simultaneous analysis of mRNAs and proteins at the single-cell level provides information about the dynamics and correlations of gene and protein expressions in individual cells, enabling comprehensive study of cellular heterogeneity and expression patterns. Here, we present a platform for high-throughput cellular indexing of mRNAs and proteins, named multi-Paired-seq, with high cell utilization, accurate molecular measurement and low cost. Based on hydrodynamic differential flow resistance, multi-Paired-seq largely improves cell utilization (>95%). Combined with pump/valve structure, cell-free antibodies and mRNAs can be removed completely for highly accurate detection. The picoliter reaction chambers allow higher detection sensitivity and lower sequencing cost. By using multi-Paired-seq, more elaborate classifications of breast cancers are identified according to multimodal measurements, and the expression correlations between mRNAs and proteins under altered conditions are quantified. Multi-Paired-seq provides multi-modal measurements at the single cell level, which offers a new tool for cell biology, developmental biology, drug discovery and precision medicine. Overall design: mRNA profiles of different cancer cell lines.
Sample: 4T1-MCF7 mixed single-omics sample RNA
SAMN22504985 • SRS10683511 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Cells were lyzed and RNAs/Proteins were captured, reverse transcribed and amplified. RNA libraries were prepared for sequencing using standard Illumina protocols.
Experiment attributes:
GEO Accession: GSM5651033
Links:
Runs: 1 run, 96.3M spots, 28.9G bases, 14.7Gb
Run# of Spots# of BasesSizePublished
SRR1653267396,331,20928.9G14.7Gb2021-10-26

ID:
17336180

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