GSM5570590: mESC to CM Day 3 rep1; Mus musculus; ATAC-seq - SRA

SRX12121242: GSM5570590: mESC to CM Day 3 rep1; Mus musculus; ATAC-seq
2 ILLUMINA (Illumina HiSeq 4000) runs: 25.4M spots, 5.1G bases, 1.9Gb downloads

Submitted by: NCBI (GEO)

Study: Cardiac differentiation of mESCs and analysis of chromatin accessibility time series

PRJNA761997 • SRP336353 • All experiments • All runs

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Assay for transposase-accessible chromatin-sequencing (ATAC-seq) was performed in a time-series of cardiac differentiation of mESCs Overall design: Two biological replicates were collected every 24 hours of cardiac differentiation of A2lox.cre mESCs from the 2i stage through day 8, in addition to day 10 and day 15 (cardiomyocyte stage). Samples were then lysed, and transposition was carried out at 37C for 30 minutes with Illumina's Nextera DNA Library Prep kit (Illumina 15028212). Libraries were generated from transposed DNA and sequenced on an Illumina HiSeq4000 instrument in the University of Chicago's Genomic Facility.

Sample: mESC to CM Day 3 rep1

SAMN21368205 • SRS10102321 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 4000

Strategy: ATAC-seq

Source: GENOMIC

Selection: other

Layout: PAIRED

Construction protocol: Nuclei were extracted by deteregent lysis. Transposition was carried out at 37C for 30 minutes. Libraries were prepared with Illumina's Nextera DNA Library Prep kit (Illumina 15028212).

Experiment attributes:

GEO Accession: GSM5570590

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 25.4M spots, 5.1G bases, 1.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR15829296	14,503,572	2.9G	1.1Gb	2024-09-02
SRR15829297	10,853,677	2.2G	798.8Mb	2024-09-02

ID:: 16040603

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