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SRX1136498: GSM1846534: ADAR1_NULL_IFN_REP1; Mus musculus; OTHER
1 ILLUMINA (Illumina MiSeq) run: 205,073 spots, 59.5M bases, 29Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Quantification of RNA editing in MEF cells of different genetic backgrounds with and without interferon treatment
show Abstracthide Abstract
We report the application of mmPCR-seq to mouse embryonic fibroblasts. We quantified RNA editing at 557 different loci using a microfluidic multiplex PCR method coupled with deep sequencing. Overall design: RT-PCR amplification of 557 loci from 6 MEF lines with and without interferon treatment to quantify RNA editing levels.
Sample: ADAR1_NULL_IFN_REP1
SAMN03980144 • SRS1027278 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina MiSeq
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: PAIRED
Construction protocol: Total RNA was extracted from cells Libraries were prepared by pooling all of the PCR products for a particular sample and barcoding them uniquely using a 15 cycle PCR reaction.
Experiment attributes:
GEO Accession: GSM1846534
Links:
Runs: 1 run, 205,073 spots, 59.5M bases, 29Mb
Run# of Spots# of BasesSizePublished
SRR2148927205,07359.5M29Mb2016-02-03

ID:
1657977

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