Instrument: Illumina NovaSeq 6000
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: The reproductive tract was removed from the dam at the abattoir. Individual fetuses, referred to as GD45, GD60, GD90 male or female (M or F) were removed from each uterine horn. No fetuses were taken from the tip of either horn or the body of the uterus. The skin and skull were cut downstream the mid-sagittal plane with a scalpel and removed near the ears. The whole brain was scooped out, weighed, measured at the longest point. Blood was collected from age-matched boars and sows. The animal was restrained using a snare and whole blood was collected with a syringe and needle from the jugular vein and put in ethylenediaminetetraacetic acid (EDTA) tubes to prevent from blood to clot. DNA from fetal brain was isolated from frozen homogenized tissue samples using AllPrep DNA/RNA Mini Kit (Qiagen, Cat No./ID: 80204) as per manufacturer's instruction. DNA from blood was isolated using QIAamp DNA Blood Mini Kit (Qiagen, Cat No./ID: 51104) as per manufacturer's instruction. Libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit as per manufacturer's instruction.