GSM5315977: decidualized hESCs, NCCD4; Homo sapiens; RNA-Seq - SRA

SRX10897757: GSM5315977: decidualized hESCs, NCCD4; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 38.2M spots, 11.5G bases, 3.6Gb downloads

Submitted by: NCBI (GEO)

Study: Next Generation Sequencing of si-NC and si-circFAM120A Transcriptomes

PRJNA730184 • SRP319952 • All experiments • All runs

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Purpose: to find the potential down-stream target gene of circFAM120A Methods: mRNA profiles of decidualized human endometrial stromal cells (hESCs) after down-regulated FAM120A and control using siRNA were generated by NGS and compared by bioinformatics analysis Results: we sequenced 26414 mRNAs in hESCs between the si-NC and si-circFAM120A groups and identified 242 differentially expressed mRNAs, between which 161 were downregulated and 81 were up-regulated in si-circFAM120A group Conclusions: Our study represents the detailed analysis of decidualized hESCs transcriptomes between si-NC and si-circFAM120A groups. Overall design: decidualized hESCs transcriptomes between si-NC and si-circFAM120A groups

Sample: decidualized hESCs, NCCD4

SAMN19222198 • SRS8990238 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted using using Trizol reagent following the manufacturer's protocol. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.

Experiment attributes:

GEO Accession: GSM5315977

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 38.2M spots, 11.5G bases, 3.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR14554385	38,229,240	11.5G	3.6Gb	2023-06-30

ID:: 14493567

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