GSM5289695: Trichoplusia ni somatic samples [F122]; Trichoplusia ni; ... - SRA

SRX10842216: GSM5289695: Trichoplusia ni somatic samples [F122]; Trichoplusia ni; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 10.8M spots, 805.3M bases, 341.8Mb downloads

Submitted by: NCBI (GEO)

Study: Ascoviruses radically optimize virion proliferation by fully cleaving host cells into viral vesicles that circulate in hemolymph

PRJNA728898 • SRP319359 • All experiments • All runs

show Abstracthide Abstract

We used RNA-Sequencing technology to analyze the transcriptomes of two ascovirus isolates, SfAV and TnAV, over three weeks. Overall design: Spodopetera frugiperda and Trichoplusia ni were infected with one of two ascoviruses, SfAV or TnAV respectively, and host tissue samples were sequenced with RNA-Sequencing technology over time in three replicates.

Sample: Trichoplusia ni somatic samples [F122]

SAMN19107206 • SRS8926230 • All experiments • All runs

Organism: Trichoplusia ni

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Hemolymph or combination of other body tissues, which from the standpoint of bulk, consisted primarily of the fat body, epidermis, tracheal matrix and midgutody tissues (referred to as somatic body tissues) were collected and homogenized in Trizol. Samples were DNAse treated and cDNA libraries were constructed. RNA libraries were prepared for sequencing using NEBNext Utra Directional RNA Library Prep kit.

Experiment attributes:

GEO Accession: GSM5289695

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 10.8M spots, 805.3M bases, 341.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR14494843	10,774,846	805.3M	341.8Mb	2021-08-19

ID:: 14381710

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