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SRX10734374: GSM5273620: H3K36me3_heart_E16_EC_cell_11; Mus musculus; ChIP-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 14,933 spots, 4.5M bases, 1.6Mb downloads

Submitted by: NCBI (GEO)
Study: Orchestrating endothelial cell fate regulation and function during multiple organs' development, stress and aging at single cell resolution
show Abstracthide Abstract
CoBATCH-supported single cell protein-bound elements profiling five tissues from Cdh5+ lineage endothelial cells (EC) collected from four stages of the mouse lifespan, discovering that intrinsic epigenetic plasticity grammarred by the TF regulome which educated by tissue microenvironment. Overall design: We applied CoBTACH to comprehensively trace Cdh5+ cells lineage for various histone modifications and TFs, obtaining total ~20,000 cells derived from 5 organs (heart, liver, lung, brain, kidney) across the lifespan of mouse. Each sample represents a single cell.
Sample: H3K36me3_heart_E16_EC_cell_11
SAMN18943825 • SRS8822152 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina NovaSeq 6000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Construction protocol: Following CoBATCH protocol. After PCR enrichment using 2X KAPA master mix, products were purified and selected by AMPure XP beads for 200-1000 bp.
Experiment attributes:
GEO Accession: GSM5273620
Links:
Runs: 1 run, 14,933 spots, 4.5M bases, 1.6Mb
Run# of Spots# of BasesSizePublished
SRR1438197314,9334.5M1.6Mb2024-04-30

ID:
14273220

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