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SRX1050401: GSM1704576: 24 h DENV1 infection Total cell lysate ribosome profiling (rep 1); Homo sapiens; OTHER
1 ILLUMINA (Illumina HiSeq 2500) run: 32M spots, 1.6G bases, 1.1Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Ribosome profiling analysis of Dengue Virus
PRJNA285961 • SRP059187 • All experiments • All runs
show Abstracthide Abstract
Dengue virus is an + strand RNA virus. We have carried our infections of human cells with Dengue and analyzed the translation, replication, and localization of the Dengue RNA. This allowed for clear definition of the life cycle of the Dengue virus inside a host cell. We also assessed the host response to Dengue virus, finding that a large fraction of the translational response is due to Interferon function. Overall design: Translational and transcriptional analysis of the cellular response to Dengue virus infection
Sample: 24 h DENV1 infection Total cell lysate ribosome profiling (rep 1)
SAMN03763468 • SRS954580 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: Pre-treatment with 180 uM cycloheximide for 30s. PBS wash. For fractionated cells, release cytosol with digitonin. Extract remaining sample with dodecyl maltoside. NEB Directional Small RNA kit PNK end polishing, linker ligation, reverse transcription, PCR amplification
Experiment attributes:
GEO Accession: GSM1704576
Links:
External link:
Runs: 1 run, 32M spots, 1.6G bases, 1.1Gb
Run# of Spots# of BasesSizePublished
SRR205298432,000,4281.6G1.1Gb2016-09-01

ID:
1525549

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