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SRX10459803: GSM5213777: wild type untagged t4 WCE; Saccharomyces cerevisiae; [Candida] glabrata; ChIP-Seq
1 ILLUMINA (NextSeq 550) run: 1.3M spots, 201.4M bases, 71.8Mb downloads

Submitted by: NCBI (GEO)
Study: Spo11 generates gaps through concerted cuts at sites of topological stress [Top2]
show Abstracthide Abstract
Meiotic recombination is essential for proper meiotic chromosome segregation and fertility, and is initiated by programmed DNA double-strand breaks (DSBs) introduced by Spo11, a eukaryotic homolog of archaeal topoisomerase VIA. Here we report the discovery of hitherto uncharacterized Spo11-induced lesions, small gaps from 34 bp to several hundred bp, which are generated by coordinated pairs of DSBs (double DSBs or dDSBs). Isolation and genome-wide mapping of the resulting fragments with single base pair precision reveals enrichment at DSB hotspots but also a widely dispersed distribution covering the entire genome. We show that Spo11 prefers to cut at a sequence motif which promotes DNA bending, indicating that bendability of DNA contributes to cleavage site choice. Moreover, fragment lengths display a ~ (10.4n+3) bp periodicity, implying that Spo11 favours cleavage on the same face of underwound DNA. Consistently, dDSB signals overlap and correlate with topoisomerase II binding sites, which points to a role for topological stress and DNA crossings in break formation, and suggests a unified model for DSB and dDSB formation, in which Spo11 traps two DNA strands. Furthermore, gaps resulting from dDSBs, an estimated 20% of all initiation events, can account for full gene conversion events that are independent of both Msh2-dependent heteroduplex repair and MutL?. Since non-homologous gap repair results in deletions, and ectopically re-integrated dDSB fragments result in insertions, dDSB formation represents a potential source of evolutionary diversity and pathogenic germ-line aberrations. Overall design: Chromatin immunoprecipitation (ChIP) of wild-type Saccharomyces cerevisiae (S. cer.) strains with myc-tagged (or untagged) Top2 from meiotic yeast cultures for 0 hrs - 4 hrs (t0 - t4) was performed and deep sequenced with Illumina. S. cer. cells were mixed with Candida glabrata (C. gla.) cells for calibration and downstream quantitative comparison. We are providing 4 immonoprecipitation (IP) and 4 corresponding whole cell extract (WCE) samples.
Sample: wild type untagged t4 WCE
SAMN18514579 • SRS8590745 • All experiments • All runs
Library:
Instrument: NextSeq 550
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Construction protocol: Cells were opened by FastPrep-24™ 5G Instrument (MP Biomedicals) followed by sonication with Bioruptor® Pico instrument. After removing the cell debris, the supernatant was used for the chromatin immuno-precipitation with anti-myc 9E11 antibody. To reverse crosslink, all the samples were incubated in 65°C overnight. DNA purification was conducted by regular phenol/chloroform/isoamyl alcohol extraction. Library preparation was performed using NEBNext® Ultra II DNA Library Prep Kit according to the manufacturer´s protocol. Amplified library DNA was purified and subjected to ~140 bp - 800 bp size selection using Agencourt AMPure XP beads (Beckman Coulter) and quantified by NanoDrop™ 3300 Fluorospectrometer and Agilent 2100 Bioanalyzer.
Experiment attributes:
GEO Accession: GSM5213777
Links:
Runs: 1 run, 1.3M spots, 201.4M bases, 71.8Mb
Run# of Spots# of BasesSizePublished
SRR140856021,342,886201.4M71.8Mb2021-06-09

ID:
13872459

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