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SRX10386184: GSM5184178: C3H10T1/2 MSCs - OB_rep2; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 52.4M spots, 15.7G bases, 1.8Gb downloads

Submitted by: NCBI (GEO)
Study: Genes differentially expressed during early osteoblast (OB) differentiation
show Abstracthide Abstract
RNA-seq analysis was performed to identify early OB-regulating genes in C3H10T1/2 MSCs. Overall design: C3H10T1/2 MSCs were left untreated (control) or induced for osteoblast differentiation (OB) for 3 days. There are 3 biological replicates for the control group and 4 for the OB group.
Sample: C3H10T1/2 MSCs - OB_rep2
SAMN18373985 • SRS8504559 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted with Trizol. Following purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperature. Then the cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the TruSeq RNA Sample Preparation v.2 (cat. no. RS-122-2001, RS-122-2002) (Illumina)
Experiment attributes:
GEO Accession: GSM5184178
Links:
Runs: 1 run, 52.4M spots, 15.7G bases, 1.8Gb
Run# of Spots# of BasesSizePublished
SRR1400925252,396,49915.7G1.8Gb2021-03-21

ID:
13704335

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